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anti human mouse il 17a  (Proteintech)


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    Structured Review

    Proteintech anti human mouse il 17a
    Anti Human Mouse Il 17a, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+mouse+il+17a/IL-17a+Antibody/pmc12278431-151-7-12
    Average 95 stars, based on 107 article reviews
    anti human mouse il 17a - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: Antisense oligonucleotides targeting IRF4 alleviate psoriasis
    Article Snippet: After quantification, the protein was loaded onto 8%, 10%, and 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis gels (25 μg/lane; Tris-Gly, #P0452S, #P0456S, and #P0459S; Beyotime). .. The gels were then electrophoretically transferred onto nitrocellulose membranes, followed by incubation with the following antibodies: IRF4 (rabbit mAb #62834, 1:1000 dilution; Cell Signaling Technology), EP300 (#sc-48343, F-4, 1:2000 dilution; Santa Cruz Biotechnology, Dallas, TX, USA), anti-human/mouse IL-17A (#26163-1, 1:1000 dilution; Proteintech, Rosemont, IL, USA), β -actin (rabbit mAb, #81115-1, 1:10,000 dilution; Proteintech), anti-human/mouse GAPDH (#60004-1, 1:10,000 dilution; Proteintech), horseradish peroxidase (HRP)-conjugated Affinipure goat anti-mouse immunoglobulin G (IgG, #SA00001-1-A, 1:20,000 dilution; Proteintech), and HRP-conjugated Affinipure anti-rabbit IgG (#7074, 1:10,000 dilution; Cell Signaling Technology). .. They were detected using electrochemiluminescence (ECL) substrate (#32134; Thermo Fisher).

    Article Title: Antisense oligonucleotides targeting IRF4 alleviate psoriasis
    Article Snippet: .. They were incubated with the following primary antibodies: anti-mouse/human IRF4 (rabbit mAb #62834, 1/1000 dilution; Cell Signaling Technology), anti-human/mouse IL-17A (26163-1-AP, 1/1000 dilution; Proteintech), and anti-rabbit IgG (1:10,000 dilution). .. Immunostaining was performed with 3,3′-diaminobenzidine solution (#P0203; Beyotime).

    Article Title: Antisense oligonucleotides targeting IRF4 alleviate psoriasis
    Article Snippet: Then, the tissues were sealed with goat serum for 30 min and permeabilized with 0.02% Triton X-100 (#HFH10; Invitrogen) for 30 min at room temperature. .. After washing, the samples were incubated with anti-human/mouse IL-17A (#26163-1, 1:1000 dilution; Proteintech) and FITC anti-mouse CD4 (#100406, GK1.5, 1:50 dilution; BioLegend) at 4 °C away from light. .. The nuclei were stained with 4′,6-diamidino-2-phenylindole (#4083S; Cell Signaling Technology).



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    Journal: iScience

    Article Title: Metformin facilitates viral reservoir reactivation and their recognition by anti-HIV-1 envelope antibodies

    doi: 10.1016/j.isci.2024.110670

    Figure Lengend Snippet:

    Article Snippet: Mouse anti-human IL-17A PE (Clone eBio64DEC17) , eBiosciences , Cat#12-7179-42; RRID: AB_1724136.

    Techniques: Purification, Virus, Plasmid Preparation, Expressing, Recombinant, Radio Immunoprecipitation, Protease Inhibitor, Western Blot, Stripping Membranes, Saline, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, DNA Purification, Cell Isolation, DC Protein Assay, Staining, Software